HepaRG™

HepaRG™ is a well-established human hepatic cell line. It is unique in its ability to generate early hepatic progenitor cells capable to fully differentiate into completely well-polarized mature adult hepatocytes. They are affordable, convenient, and available either as progenitor cells to be expanded and differentiated in-house (HPR101), or as cryopreserved, fully differentiated and ready-to-use hepatic cells (HPR116).

HepaRG™ cell line

HepaRG™ is a well-established hepatic cell system. It is unique in its ability to generate early hepatic progenitor cells capable to fully differentiate into well-polarized mature adult human hepatocytes.

Main characteristics of HepaRG™ cell line

HepaRG™ is an immortal cell line with 4 main features:

  • Full array of functions, responses, and regulatory pathways of primary human hepatocytes including the biotransformation enzymes
  • Forms functional bile canaliculi-like structures
  • Has the potential to express many properties of stem cells: high plasticity & transdifferentiation
Available products
  • HepaRG cells are available either as progenitor cells to be expanded and differentiated in-house, or as cryopreserved, fully differentiated and ready-to-use hepatic cells. HepaRG™ cell line represents a robust and valuable surrogate for primary hepatocytes. It is affordable, available worldwide, and convenient with high interassay reproducibility compared to primary human hepatocytes.
  • Cell culture media and appropriate supplements for different uses are also available.
Tutorials and support

Find tips about cell thawing, viability measurement, seeding and maintenance in our detailed protocol. For best practices of cell culturing and maintaining, watch our tutorial videos and ask for customized training sessions and technical assistance including special packages (emails, calls, and filmed live sessions).

Examining target interactions and mechanisms for your cell line selection and use

Dynamic gene expression profiling of the HepaRG cell line

Start using the cell line

You can order the HepaRG™ cells, media and supplements online. Our experts can offer advice on the appropriate products for your applications.