The cells can be purchased as fully differentiated cells or undifferentiated growth-stage cells.
Fully differentiated cells: HPR116
Fully differentiated cells have characteristics closely related to those of primary hepatocytes. Advantages:
- Ready and easy-to-use model;
- High interassay reproducibility;
- Time-saving and efficiency;
- Suitable for high-throughput applications;
- Functionality across multiple applications;
- Cost-effective compared to primary human hepatocytes;
- No need of particular equipment;
Undifferentiated growth-stage cells: HPR101
Undifferentiated growth-stage cells can be grown in-house to set-up a master and/or working bank or further differentiated following the recommended procedures. Advantages:
- Possibility of cell line manipulation such as transfections, gene editing, etc;
- A proliferating cell population, adequate for some particular applications (e.g., Micronucleus test);
- Karyotypic stability: can be grown and split up till eight times when done according to the recommended protocols and using appropriate medium supplements;
- Possibility of cell line amplification
- Cells in proliferation stage tolerate some viral infections more easily than differentiated, adult hepatocytes.
HPR101 - Undifferentiated HepaRG™ cells
HPR101 are cryopreserved undifferentiated HepaRG cells at passage 12. These cells are intended to be expanded and differentiated in-house. The undifferentiated HepaRG cell line is available only after a license agreement made with Biopredic International (for profit customers) or under a MTA made with Biopredic International (for non-profit customers that are not funded neither in full nor in part by a profit organization).
HPR116 - Differentiated HepaRG™ cells
HepaRG™ cells, catalog number HPR116 are cryopreserved after differentiation using a proprietary process developed by Biopredic International. A few days after cell thawing and culturing, a coculture of hepatocytes and of biliary epithelial-like cells is obtained.
Media, supplements and plates
The plasticity of HepaRG cells is a property which can easily lead to cells phenotypically different from those described in the literature when HepaRG cells are not cultured and maintained according to the recommendations and under the appropriate conditions.
We recommend using our cell culture media and supplements with containing very carefully selected components. The formulation of our cell culture media and supplements ensures a correct culture of HepaRG cells leading to fully differentiated hepatocyte-like cells.
Our media rely on supplements (called ADDs) adapted to the phase stage of the HepaRG™ cell culture and to the usage intended use of the cells. Those ADDs are stored frozen and added to a basal hepatic cell culture medium.