HPR116NS - Differentiated HepaRG™ No-Spin cells
HepaRG® cells, HPR116NS is a new format of the HPR116 with a freezing process that allows direct thawing and seeding of cryopreserved differentiated cells without the need for post-thaw washing, centrifugation and counting steps. A few days after thaw and culture, the cells will form a coculture of hepatocytes and of biliary epithelial-like cells. The cells are marketed by Lonza exclusively (except in Japan).
|Origin||Liver tumor of a patient suffering from hepatocarcinoma and hepatitis C infection|
Absence of hepatitis B, hepatitis C and HIV1 viruses checked by PCR on the cell suspension.
Caution: although controls are performed, human material has to be considered as potentially dangerous. Take maximum care in order to protect yourself and your colleagues.
HepaRG® cells, catalog number HPR116 are cryopreserved after differentiation using a proprietary process developped by Biopredic International. A few days after thaw and culture, the cells will form a coculture of hepatocytes and of biliary epithelial-like cells.
Gripon P. et al. Proc. Natl. Acad. Sci. 99, 15655-15660, 2002.
|Packaging||1 mL vial with 8 x 10E+6 viable cells|
One vial will fully seed one 96-well plate (72 000 cells/well)
One vial will fully seed 16 wells of one 24-well plate (480 000 cells/well)
|CYP genotyping data||Obtained in collaboration with N. Picard at Limoges hospital (France) using a validated TaqMan allelic discrimination assay (ABI PRISM 7000 Sequence Detection System, Applied-Biosystems).|
SNPs: Single Nucleotide Polymorphisms