Cell seeding Catalog Number HPR116

2D cultures

1. Seed the HepaRG™ cells into flat bottom multi-well plate(s) or flask(s) according to the table:

   Culture plate format	Number of viable cells per well/flask	Volume per well/flask	Cells per ml
   25cm2 flasks	5.2 x 106	5 ml	1.04 x 106
   6-well plate	2 x 106	2 ml	1 x 106
   12-well plate	0.8 x 106	1 ml	0.8 x 106
   24-well plate	0.48 x 106	0.5 ml	0.96 x 106
   48-well plate	0.16 x 106	0.2 ml	0.8 x 106
   96-well plate	0.072 x 106	0.1 ml	0.72 x 106
   384-well plate	0.024 x 106	0.04 ml	0.6 x 106

2. Distribute the cells equally along the plate surface and control the homogeneity of the cell distribution.
3. Incubate cells at 37 ˚C, 5%CO2 and saturating humidity