Measurement of viability after single application: MTT test Step 3 (Day 2)

Prediskin - Day 2A
  1. Remove the skin disks using forceps, exerting pressure to release the isopropyl alcohol as much as possible, then transfer the explants to a container reserved for biological waste.
  2. Homogenize the solutions, take 2 x 200µL per well then transfer them to a 96-well plate (s), additionally provide on one of the 2 * 200µL plates for the blanks
  3. Reading the optical density of the 96-well plate (s) at 590 nm against isopropyl alcohol blanks.
  4. For OD > 3.0, provide a 1/2 dilution in isopropyl alcohol and take a reading against new isopropyl alcohol blanks (2 * 200 * µL).
Prediskin - Day 2

<< Back to Step 2


SaferWorldbyDesign integrates in silico and in vitro methods to develop standard and premium Integrated Approaches to Testing and Assessment (IATAs), including methods that support ingredient and formulation screening and testing, product design and the preparation of Regulatory Dossiers.


Edelweiss Connect GmbH is registered as a limited company with Handelsregisteramt of Kanton Basel-Stadt

Swiss ID (UID) number: CHE-114.414.615

Copyright | SaferWorldbyDesign 2022